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Comparison of two multiplex (biochip) methods for determination of specific IgE against molecular defined allergens
Velčovská, Denisa ; Andrýs, Ctirad (advisor) ; Jankovičová, Karolína (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Subject of study: Bioanalytical laboratory diagnostics in health care Author: Bc. Denisa Velčovská Supervisor: prof. RNDr. Ctirad Andrýs, Ph.D. Title of diploma thesis: Comparison of two multiplex (biochip) methods for determination of specific IgE antibodies against food allergens in group of patients suffering from atopic dermatitis This thesis deals with the comparison of two multiplex (biochip) methods ALEX and ISAC in the determination of specific IgE antibodies against selected allergens in patients with atopic dermatitis. The aim of this thesis is to statistically compare the two methods and perform an evaluation of the data obtained, to assess whether the two methods provide similar results and are interchangeable, or whether a statistically significant difference can be observed. This diploma thesis mainly focuses on inhalant allergens of moulds, mites, house dust mites and animal allergens. The theoretical part of this diploma thesis is focused on allergies and their clinical forms. It also describes allergens, their nomenclature, biochemical structure and methods used to diagnose allergic inflammation. In the experimental part, the basic statistics of the cohort, which includes 96...
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